The Effect of Mucosidosis (Cystic Fibrosis) and Bacterial Factors on CFTR (CF Transmembrane Conductance Regulator) Channel Efficiency

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Mucocidosis (cystic fibrosis) is the most common autosomal recessive inherited multisystem disease with a very deadly and dangerous outcome due to mutations and sequential mutations in the gene regulating the transmission of cystic fibrosis membrane (CFTR) leading to the chloride channel. And the CFTR protein becomes unhealthy and destructive [1,2]. As a result of the collection and collection of mucus that is not sufficiently hydrated and hydrophilic, it affects various basic organs, mainly lungs, and airways, pancreas, liver, bile ducts and intestines. This leads to inflammation and infection, fibrosis, and gradual tissue destruction [3-5]. Respiratory failure and subsequent problems have been cited as one of the leading causes of death. However, more than 30 years of molecular description A major defect in CFTR causes CF, a remarkable success in the long-term prognosis of people with CF. This breakthrough is very active in the spirit of collaboration and networking of the CF research community, and internationally, with the creation of a multidisciplinary clinical CF team that implements evidence in various aspects of standard care with the CF patient has been obtained. Rath et al. [5]. In their study found that several bacteria and fungi that cause organisms are also known as urinary tract infections (UTIs) that may occur in more than 50% of the population and are more common in women [5-8]. Urinary tract infection is one of the few and most limited bacterial infections approved by the laboratory, with an intrinsic limit of at least 3-4 days for the results of antibiotic susceptibility patterns to produce bacteria that cause bacteria in a urine sample. The experimental treatment is started according to the antibiotic inhibition program due to pain under radiation under the abdomen and inability to perform normal activities. This prescribed antibiotic often controls the disease. However, when there is an infection with a multidrugresistant bacterium (MDR), it is most often transmitted to cystitis and then to pyelonephritis [8,9]. Due to this problem, a clinician inadvertently prescribes an antibiotic with It prescribes a wide range, the generation, which insidiously considers the resistance to the same antibiotic in the world as a pathogen [10,11].

In another study, Chakotiya et al. [8] significantly increased the incidence of lung infections caused by Pseudomonas aeruginosa strains, which are classified as multidrug-resistant, mainly due to the relative inadequacy of potent chemotherapy. And is attributed to complication [9,10]. The present study was performed to evaluate the antimicrobial activity of the aqueous-alcoholic extract of Glycyrrhiza glabra against P.aeruginosa causing lung infection in Swiss albino mice. This study included evaluating the lethal dose of P. aeruginosa in Swiss albino mice and analyzing disease manifestations that included bacteria, hypothermia, weight loss, and other parameters for 48 hours of infection. Physical manifestations of infected mice showed a significant decrease in body temperature from 29.58 29 29 °C (in 48 hours) to 38.81 3 3.33 °C (0h) and 30% weight loss at the end of the study. In addition, the effect of G. glabra extract against lung infection caused by lethal dose was calculated using bacteremia, histopathology, and radiological analysis. Bacterial load showed that 2.230-30 Log10CFU/ml on day 7 had a significant decrease in bacterial load compared to day 1 when Log10CFU/ml bacterial load decreased by 3.32 32.3. Histopathological results have shown that scattered and fragmented accumulation of inflammatory cells in the alveolar space has increased and also infiltrations have been observed in all parts of the lungs of infected mice [5,8]. In the treated animal’s group, improved pulmonary histology with the discharge was less seen at D1 dose (20mg/kg) and disappeared at D2 dose (80mg/ kg). This study clearly states that G.glabra extract is effective against lung infection caused by P. aeruginosa at a dose of 80mg/ kg. LCMS results showed that this extract contained glycyrrhizin, stigmasterol, and ergosterol, Liquocalcon, and guanidine. The present study is expected to further exploit the biomedical properties of this extract in preparing a strong diet against such threatening pathogens [10,12,13].

The resistance of multi drugs presented and evaluated by Pseudomonas aeruginosa to antibiotics was also reflected in its tendency to produce biofilms, cell membrane modifications, and antibacterial drugs. The study of the activity of Glycyrrhiza glabra and one of its pure compounds, glycyrrhizic acid against P. aeruginosa and their mechanism of action in terms of the effect on membrane permeability, flow activity, and biofilm formation has been determined [14,15]. Minimum inhibitory concentration with the dilution method and the amount of dilution of broth in the casting medium has been determined [16,17]. The minimum antibacterial concentration on the agar plate has been investigated. The MIC of the extract and glycyrrhizic acid were 200 and 100μgml (-1), respectively. The MBC for the extract and glycyrrhizic acid is 800 and 400μg, respectively. The effect of time-dependent homicide has also been estimated. Flow cytometric analysis was performed to determine the effect of extract and glycyrrhizic acid at 2×MIC on various physiological parameters using staining methods and comparison with a standard (antibiotic). The growth of P.aeruginosa was significantly inhibited by extracts and pure compounds. Plant extracts and glyceric acid were also effective in targeting the physiological parameters of bacteria, including cell membrane permeability, flow activity, and biofilm formation. This study reports the anti-pseudomonal function of Glycyrrhiza glabra and one of its compounds and provides insight into their function [8,17,10].

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